Pubblicazione scientifica

Detection of bacterial-reactive natural IgM antibodies in desert bighorn sheep populations

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Ecoimmunology is a burgeoning field of ecology which studies immune responses in wildlife by utilizing general immune assays such as the detection of natural antibody. Unlike adaptive antibodies, natural antibodies are important in innate immune responses and often recognized conserved epitopes present in pathogens. Here, we describe a procedure for measuring natural antibodies reactive to bacterial antigens that may be applicable to a variety of organisms. IgM from desert bighorn sheep plasma samples was tested for reactivity to outer membrane proteins from Vibrio coralliilyticus, a marine bacterium to which sheep would have not been exposed. Immunoblotting demonstrated bighorn sheep IgM could bind to a variety of bacterial cell envelope proteins while ELISA analysis allowed for rapid determination of natural antibody levels in hundreds of individual animals. Natural antibody levels were correlated with the ability of plasma to kill laboratory strains of E. coli bacteria. Finally, we demonstrate that natural antibody levels varied in two distinct populations of desert bighorn sheep. These data demonstrate a novel and specific measure of natural antibody function and show that this varies in ecologically relevant ways.

DATA

Data di pubblicazione: 29/06/2017

AUTORI

DUGOVICH BRIAN S
PEEL MELANIE J
PALMER AMY L
ZIELKE RYSZARD A
SIKORA ALEKSANDRA E
BEECHLER BRIANNA R
JOLLES ANNA E
EPPS CLINTON W
DOLAN BRIAN P

ENTE DI AFFERENZA

DEPT BIOMEDICAL SCIENCES COLLEGE OF VETERINARY MEDICINE OREGON STATE UNIV CORVALLIS OREGON USA
DEPT PHARM SCIENCES COLLEGE OF PHARMACY OREGON STATE UNIV CORVALLIS OREGON USA
DEPT FISHERIES & WILDLIFE COLLEGE OF AGRICULTURAL SCIENCES OREGON STATE UNIV CORVALLIS OREGON USA
INTL NUTRITION INC US

RIVISTA

PLoS ONE, 12(6), e0180415

IMPACT FACTOR

2,870

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