Pubblicazione scientifica

Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Immune cells sense and react to a multitude of factors including both host and microbe-derived signals. Understanding how cells translate these cues into particular cellular behaviors is a complex yet critical area of study. We have previously shown that both neutrophils and macrophages are important for controlling the fish pathogen Streptococcus iniae. Here, we report both host and bacterial determinants leading to the formation of organized macrophage aggregates as part of the host inflammatory response in a subset of infected larvae. Streptococcal capsule was a required signal for aggregate formation. Macrophage aggregation coincided with NFκB activity, and the formation of these aggregates is mediated by leukotriene B4 (LTB4) produced by neutrophils. Depletion, inhibition, or genetic deletion of leukotriene A4 hydrolase (Lta4h), which catalyzes the last step in LTB4 synthesis, resulted in the absence of macrophage aggregation. Larvae with impaired neutrophil function also had impaired macrophage aggregation; however, aggregate formation was partially rescued with the addition of exogenous LTB4. Neutrophil-specific expression of lta4h was sufficient to rescue macrophage aggregation in Lta4h-deficient larvae and increased host survival following infection. In summary, our findings highlight a novel innate immune response to infection in which specific bacterial products drive neutrophils that modulate macrophage behavior through eicosanoid signaling.

DATA

Data di pubblicazione: 28/06/2017

AUTORI

VINCENT WILLIAM J B
HARVIE ELIZABETH A
SAUER JOHN-DEMIAN
HUTTENLOCHER ANNA

ENTE DI AFFERENZA

MICROBIOLOGY DOCTORAL TRAINING PROGRAM DEPT MEDICAL MICROBIOLOGY & IMMUNOLOGY UNIV WISCONSIN MADISON MADISON WI USA
DEPT MEDICAL MICROBIOLOGY & IMMUNOLOGY UNIV WISCONSIN MADISON MADISON WI USA
DEPT PEDIATRICS UNIV WISCONSIN MADISON MADISON WI USA
DUKE UNIV US

RIVISTA

PLoS ONE, 12(6), e0179574

IMPACT FACTOR

2,870

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