Acquacoltura Pubblicazione scientifica Characterization of prophages of Lactococcus garvieae FILONE TECNOLOGICO TEMA produzione e raccolta RIASSUNTO This report describes the morphological characterization and genome analysis of an induced prophage (PLg-TB25) from a dairy strain of Lactococcus garvieae. The phage belongs to the Siphoviridae family and its morphology is typical of other lactococcal phages. A general analysis of its genome did not reveal similarities with other lactococcal phage genomes, confirming its novelty. However, similarities were found between genes of its morphogenesis cluster and genes of Gram-positive bacteria, suggesting that this phage genome resulted from recombination events that took place in a heterogeneous microbial environment. An in silico search for other prophages in 16 L. garvieae genomes available in public databases, uncovered eight seemingly complete prophages in strains isolated from dairy and fish niches. Genome analyses of these prophages revealed three novel L. garvieae phages. The remaining prophages had homology to phages of Lactococcus lactis (P335 group) suggesting a close relationship between these lactococcal species. The similarity in GC content of L. garvieae prophages to the genomes of L. lactis phages further supports the hypothesis that these phages likely originated from the same ancestor. DATA Data di pubblicazione: 12/05/2017 AUTORI ERACLIO GIOVANNI FORTINA MARIA G LABRIE SIMON J TREMBLAY DENISE M MOINEAU SYLVAIN RIVISTA Scientific Reports, 7, 1856