Pubblicazione scientifica

Identification and validation of sRNAs in Edwardsiella tarda S08

FILONE TECNOLOGICO	TEMA
produzione e raccolta

RIASSUNTO

Bacterial small non-coding RNAs (sRNAs) are known as novel regulators involved in virulence, stress responsibility, and so on. Recently, a lot of new researches have highlighted the critical roles of sRNAs in fine-tune gene regulation in both prokaryotes and eukaryotes. Edwardsiella tarda (E. tarda) is a gram-negative, intracellular pathogen that causes edwardsiellosis in fish. Thus far, no sRNA has been reported in E. tarda. The present study represents the first attempt to identify sRNAs in E. tarda S08. Ten sRNAs were validated by RNA sequencing and quantitative PCR (qPCR). ET_sRNA_1 and ET_sRNA_2 were homolous to tmRNA and GcvB, respectively. However, the other candidate sRNAs have not been reported till now. The cellular abundance of 10 validated sRNA was detected by qPCR at different growth phases to monitor their biosynthesis. Nine candidate sRNAs were expressed in the late-stage of exponential growth and stationary stages of growth (36~60 h). And the expression of the nine sRNAs was growth phase-dependent. But ET_sRNA_10 was almost expressed all the time and reached the highest peak at 48 h. Their targets were predicted by TargetRNA2 and each sRNA target contains some genes that directly or indirectly relate to virulence. These results preliminary showed that sRNAs probably play a regulatory role of virulence in E. tarda.

DATA

Data di pubblicazione: 07/03/2017

AUTORI

SUN YUYING
ZHANG JIQUAN
QIN LEI
YAN CUI
ZHANG XIAOJUN
LIU DANDAN

ENTE DI AFFERENZA

COLLEGE OF MARINE LIFE & FISHERIES HUAIHAI INST TECH LIANYUNGANG CHINA
JIANGSU MARINE RESOURCES DEV RSCH INST LIANYUNGANG CHINA
CO INNOV CTR JIANGSU MARINE BIO IND TECH HUAIHAI INST TECH LIANYUNGANG CHINA
KEY LAB OF EXPERIMENTAL MARINE BIOLOGY INST OCEANOLOGY CHINESE ACAD SCIENCES QINGDAO CHINA
UNIV PUTRA MALAYSIA MALAYSIA

RIVISTA

PLoS ONE, 12(3), e0172783

IMPACT FACTOR

2,870

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